Microstructured multi-well plate for three-dimensional packed cell seeding and hepatocyte cell culture
نویسندگان
چکیده
منابع مشابه
three-dimensional (3d) cell culture conditions, present and future improvements
conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...
متن کاملThree-dimensional microwell arrays for cell culture.
We propose the concept of three-dimensional (3D) microwell arrays for cell culture applications and highlight the importance of oxygen diffusion through pores in all three dimensions to enhance cell viability.
متن کاملThree-Dimensional Cell Seeding and Culture in Novel Radial-Flow Perfusion Bioreactor
Tissue engineering has been developed as a new approach in regenerating tissues and organs using cells and scaffolds. An important element in successful tissue engineering in vitro is a suitable bioreactor system (Freed and Vunjak-Novakovic 1997). In general, some types of bioreactors have been used for cell culture on polymer scaffolds, namely spinner flasks, rotating vessels, and perfusion sy...
متن کاملDevelopment of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture
Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneu...
متن کاملThermosensitive polymeric matrices for three-dimensional cell culture strategies.
A completely new strategy for cell culture focusing on the design of three-dimensional (3D) smart surfaces by supercritical fluid technology has been developed. This approach might overcome the limitations on cell expansion and proliferation of currently existing techniques. An alternative technology, based on supercritical carbon dioxide, was used to polymerize poly(N-isopropylacrylamide) (PNI...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomicrofluidics
سال: 2014
ISSN: 1932-1058
DOI: 10.1063/1.4892978